plant cells have the potential to grow as a plant. This potential of the plant cell is called to be totipotency. Due to that potential any plant part can develop a plant as a whole. These plant tissue culture is a technique of growing plant cells, tissues or organs in an artificially prepared nutrient medium under an ascptic condition in the laboratory.
Historical background
Haberlandt (1896) was the first person to culture Bolated vegetative calls of higher plants in simple nutrient solutions. Although Haverlandt was able to maintained the cells in the nutrient media the cell division was not recorded until much later.
Embryos were the first plant tissue to be successfully cultured in vitro on artificial medium. The first result of successfully in vitro culture was by Hanning (1904) who grew plants under aspetic conditions relatively mature embryos of Raphanus, R. landra, R. sativus and cochleasia danica (Brassicacesae) on Tollens medium with sugars, amino acids and plants extracts and obtained transplantable seedings.
The first major breakthrough in the field of plant tissue culture was made in 1922 when Robbins (USA) and Kotte (a student achieved success in growing isolated roots tips. white (1935) grew cultures of tomato root tips for a longer period ( more than 20 weeks) using sucrose, inorganic salts and yeast extract.
Further development in tissue culture of bio technology was made in 1934 by Gantheret of France who culture cambium cells of some tree species (populus higra) on knop's solution. skoog (1944) and skoog and tusi (1951) reported that in tobacco pith tissue cultures the addition adenine and high level of phosphate increased callus growth and bud formation in the presence of IAA. Musrashighe (1961) demonstrarted the technique in vitro culture of propagation of various plant species. Gaha and Maheshwari (1964) first of all successfully reported the anther culture of Datura innoxia
0 comments:
Post a Comment